NON-INVASIVE ELECTROMECHANICAL CELL-BASED BIOSENSORS FOR IMPROVED
INVESTIGATION OF 3D CARDIAC MODELS
Tématický okruh: Varia | |
| Typ: Ústní sdělení - technik , Číslo v programu: 296 | |
| Caluori G.1, Přibyl J.2, Pešl M.1, Jelinkova S.3, Rotrekl V.3, Skladal P.4, Raiteri R.5 1 ICE, FNUSA-ICRC, Brno, 2 Nanobiotechnology, CEITEC MU, Brno, 3 Biology Department, MUNI, 4 Nanobiotechnology, CEITEC MU, 5 DIBRIS, University of Genoa, Genoa, Italy | |
Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric / nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’drug response testing and DMD characterization.The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explainedthe observed effects despite variability of biological samples. The | |
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